454-pyrosequencing analysis of normal gastric mucosal microbiota
نویسندگان
چکیده
منابع مشابه
Non-culture Dependent Survey of the Microbiota of the Glassy-winged Sharpshooter Using 454 Pyrosequencing
The glassy-winged sharpshooter (GWSS; Homalodisca vitripennis) is an invasive pest that has spread across the southern and western United States. It is highly polyphagous, feeding on at least 100 species in 31 families (Hoddle et al., 2003; Turner and Pollard, 1959), and a voracious feeder, having been known to consume up to 100 times its weight in xylem fluid daily. This insect is a vector of ...
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Patients with acquired immunodeficiency syndrome (AIDS) are at high risk of opportunistic infections. Oral manifestations have been associated with the level of immunosuppression, these include periodontal diseases, and understanding the microbial populations in the oral cavity is crucial for clinical management. The aim of this study was to examine the salivary bacterial diversity in patients ...
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MOTIVATION Throughout the recent years, 454 pyrosequencing has emerged as an efficient alternative to traditional Sanger sequencing and is widely used in both de novo whole-genome sequencing and metagenomics. Especially the latter application is extremely sensitive to sequencing errors and artificially duplicated reads. Both are common in 454 pyrosequencing and can create a strong bias in the e...
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The paper describes a novel approach for filtering duplicate reads from 454 pyrosequencing data. This problem is motivated by the need of reduce sequencing errors and artifically duplicated reads in some applications such as de-novo whole genome sequencing or metagenomics. Existing solutions are often based on nucleotide sequences, while raw flowgram values, which contain additional information...
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[This corrects the article on p. 115 in vol. 22, PMID: 28698866.].
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ژورنال
عنوان ژورنال: World Chinese Journal of Digestology
سال: 2013
ISSN: 2219-2859
DOI: 10.11569/wcjd.v21.i13.1250